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The 2 protein bands from forty kDa to 30 kDa ended up absent immediately after 24 h incubation at forty five °C. For the remainder of the bands, many concentrations of all three proteases retained protein bands akin to their first molar masses (Determine 6a,b). The existence of bands at very low molecular pounds in fish roe before hydrolysis was unexpected. Within the report by Ghelichi et al. [8], minor peaks of a comparatively low molecular weight variety (3–14 kDa) ended up noticed in defatted carp roe homogenate, probably as a consequence of an autolytic course of action occurring in fish roe and/or even the defatting system leading to the formation of small-molecular-pounds peptides.
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